Empower Labs to Analyze Fecal Contamination Effectively with a LIMS for Water & Wastewater Testing

LIMS for Water & Wastewater Testing - Streamline Fecal Testing

Close to 2 billion people globally lack access to safe drinking water. Access to safe drinking water is not only a fundamental prerequisite for good health but also a basic human right. Ensuring global access to safe drinking water is a critical responsibility undertaken by the World Health Organization (WHO) and the United Nations Children’s Fund (UNICEF). WHO and UNICEF, as global leaders responsible for ensuring good public health and child welfare, play a crucial role in monitoring access to safe drinking water on an international scale. To assess global progress, they employ the “use of an improved source” as the primary indicator. While monitoring the use of improved sources provides valuable insights into access to water infrastructure, it does not directly address the quality of the water being consumed. In other words, an improved source might still deliver water with contaminants such as fecal matter.

Fecal contamination is often associated with unsanitary conditions and poor hygiene. However, it can be found in unexpected places and poses serious health risks to individuals and communities. In this blog, we will explore what fecal contamination is, its potential sources, and the importance of testing for it to safeguard public health.

What is Fecal Contamination?

Fecal contamination occurs when fecal matter containing harmful bacteria, viruses, and parasites is present in water, food, or surfaces. It is a major public health concern because it can harbor a wide range of pathogens, including bacteria like E. coli, Salmonella, and Campylobacter; viruses such as norovirus and hepatitis A virus (HAV); and parasites like Giardia and Cryptosporidium. These microorganisms can cause severe diarrhea, vomiting, abdominal pain, and dehydration, especially in vulnerable populations like young children, the elderly, and those with weakened immune systems.

Sources of Fecal Contamination

To effectively address fecal contamination, it is crucial to identify its sources. Understanding where the contamination originates allows authorities to implement targeted solutions to prevent its spread. Here are some common sources of fecal contamination:

  • Sewage and Wastewater: Inadequate or malfunctioning sewage treatment systems can release untreated human waste into water bodies, leading to contamination.
  • Agricultural Runoff: Excessive use of fertilizers and improper waste management in agriculture can cause fecal matter from livestock to contaminate nearby water sources.
  • Animal Waste: Pets and wild animals can also contribute to fecal contamination, especially in urban areas and recreational spaces.
  • Faulty Septic Systems: Malfunctioning septic tanks can release untreated sewage into the soil and groundwater, potentially contaminating wells and drinking water sources.

How to Test for Fecal Contamination in Water Sources

Several methods and techniques are used to detect the presence of fecal matter and associated pathogens in water. Here’s a general overview of how to test for fecal contamination in water:

1. Collect Water Samples: To begin the testing process, you need to collect water samples from the source you want to analyze. It could be a well, river, lake, tap water, or any other water body. It’s important to use sterile containers to prevent any external contamination during the sampling process.

2. Preserve the Samples: To preserve the integrity of the water samples, they should be stored at a low temperature (usually 4°C) and analyzed as soon as possible. If an immediate analysis is not possible, the samples can be frozen until testing can be performed.

3. Microbiological Analysis: It is the most common method used to test for fecal contamination in water. It involves detecting the presence of specific indicator organisms or pathogens that are commonly associated with fecal matter.

a. Coliform Bacteria Test: Coliform bacteria are commonly used as indicators of fecal contamination. The most widely used coliform bacteria is Escherichia coli (E. coli). The presence of coliform bacteria, especially E. coli, indicates the potential presence of fecal contamination and pathogenic organisms.

b. Membrane Filtration: In this method, a water sample is passed through a special filter that traps the bacteria present in the water. The filter is then placed on a growth medium in a petri dish, and after an incubation period, colonies of coliform bacteria, including E. coli, can be counted.

c. Enzyme Substrate Tests: Some rapid tests use enzyme substrates that react with specific enzymes produced by coliform bacteria, producing visible color changes or fluorescence when positive for coliforms. These tests provide faster results compared to traditional culture-based methods.

4.  Polymerase Chain Reaction (PCR): PCR is a molecular biology technique employed for identifying the existence of particular DNA sequences originating from target organisms, including pathogens like E. coli and other waterborne pathogens. PCR can provide highly sensitive and specific results and can identify pathogens that  culture-based methods may not detect.

5. Testing for Specific Pathogens: In addition to coliform bacteria, it may be essential to test for specific waterborne pathogens like Giardia, Cryptosporidium, norovirus, and other enteric viruses. These pathogens can cause severe illnesses, especially in individuals with compromised immune systems.

6. Chemical Analysis: In some cases, a chemical analysis may be performed to detect the presence of certain chemical contaminants associated with fecal contamination, such as nitrates and phosphates. These chemicals can also indicate the presence of human or animal waste in the water.

The methods mentioned above, such as microbiological analysis and PCR, provide valuable information about the presence of fecal matter and associated pathogens. Regular and accurate testing is essential to identify and address contamination issues promptly, safeguarding public health and the environment.

How Does a LIMS for Water & Wastewater Testing Support Fecal Testing?

A LIMS (Laboratory Information Management System) supports fecal testing by streamlining sample tracking, data management, and result reporting, ensuring efficient and organized handling of fecal samples in a laboratory. A cloud-hosted LIMS for water and wastewater testing facilitates the integration of various testing methods and quality control measures, enhancing the accuracy and reliability of fecal testing procedures.


Fecal contamination is a serious health concern that should not be underestimated. The presence of harmful pathogens in water, food, and surfaces can lead to outbreaks of waterborne diseases and other gastrointestinal illnesses. Identifying the sources of fecal contamination is crucial to implementing effective preventive measures. Regular testing for fecal contamination helps ensure the safety of water sources, food supplies, and public spaces, safeguarding the health and well-being of individuals and communities. An advanced LIMS for water & wastewater testing bolsters labs engaged in fecal testing. It minimizes the turnaround time, ensures precision through quality control protocols, and expedites the reporting of test results to stakeholders.

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